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Hello all,
I have a question regarding cell culture.
I am growing adherent cells and after treating them with chemicals f.i. to induce cell cycle arrest, I can see that some cells remain adherent and some go into suspension.
Trypan blue assay shows that the floating ones are not dead. So are they still alive but in the apoptotic pathway or can cell cycle arrest have an effect in their adherence maybe?? And generally if adherent cells go into suspension are they necessarily dead/ going to die??
Western shows different results in the adherent and floating cells so I am not sure what to trust.
Any suggestions or ideas would be very welcome!
Thanks a lot!!
