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hsbiopeep

Member Since 31 Mar 2012
Offline Last Active May 21 2012 01:54 PM
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#133454 Extracting DNA from fruits and Vegetables

Posted pito on 24 April 2012 - 11:35 PM

View Posthsbiopeep, on 24 April 2012 - 04:14 PM, said:

I was given this topic by my biology teacher but I am not sure how I will be able to observe the differences between fruits and vegetables.

Will certain bands only be present in fruits rather than vegetables?

What I am trying to ask is how should I try to observe the differences between fruits and vegetables?

Its all you can do: use the RE and then watch what it gives on the gel.
I have never done this before, so not sure what to expect.
But indeed: the more the DNA is different, the more difference you will see in the bands...

Just test a few different kinds of fruits and test some vegetables, put them on gel (more then 1 sample per fruit/vege) and check the differences.
Check fruits vs fruits and fruits vs vegetables and vegetables vs vegetables

Also: be sure to test the tomato.. (look up: Nix v. Hedden) and see for yourself whether the court was right or not...
(be sure to try to find a definition on what a fruit or vegetable is..)
(==> also starting from this "definition" you should be able to find out whether there would be a big difference or not and what might be the cause of this difference on DNA level..)


#133302 Extracting DNA from fruits and Vegetables

Posted pito on 22 April 2012 - 11:26 PM

View Posthsbiopeep, on 22 April 2012 - 04:04 PM, said:

View Postpito, on 15 April 2012 - 10:32 AM, said:

Sure why not.
Hae III wiill cut a lot, so its possible you will get very small pieces of DNA.


So is it good to have small pieces of DNA or not?
Well.. it depends...
It all depends on what you want.

What I wanted to say with it is that you will get many small pieces.. so you will have a gel with many pieces.. rather then a few.. It could be more difficult to see differences ...
Also: small pieces means: watch out with the time you let the gel run.. the pieces could migrate off the gel.


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