Yep, I understand the concept. So basically I'm SOL to compare these cell lines? If I seeded at the appropriate density for the slowest growing line, my fastest one will be over confluent at 72 hours - unless I only split it, but that also seems invalid.
bob1 - Growth rate only matters in that I need a sufficient number of cells to assay at the end of the treatment. I think I would have to seed the cell lines at different densities (i.e. the slowest growing at a higher density and the fastest growing at a lower density). Is that valid?
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