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My only comment is that you need to put the add-ons on the 5' end of the reverse primer always! You could copy and paste what you have written above into a primer ordering site and it would give you the correct oligo at the end, it's just that you have written the 5' and 3' at the wrong ends.
#131235 Primers for Introduction of new restriction sites to a vector
Posted
on 18 March 2012 - 04:41 PM
