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Need your help to find a solution for my problem to indicate significant differences in a bar chart plot. The data of the statistical test is available in the following format:
Bar1-bar2:significant
Bar1-bar3:not significant
I want to mark significant differences between two bars with different letters (like bar1:a and bar2: b. If there is no significant differences between two bars they get the same letter (like bar1:a and bar3:a). Sort the right letters to the bars gets much more complex when the number of bars increases. I have several hundreds of statistical comparisons here and have to find a computer program that can generate the letters from the data for me.
Anybody an idea which programme can help me?
I have added an example plot with letter-coded significant differences to illustrate what I want to do.
*** If any one can help me to obtain a good reference material that guide to Interpretation and analysis of biological research data would be much grateful.
Attached a sample e.g.
Akila Wijerathna Yapa
Member Since 12 Mar 2012Offline Last Active Dec 26 2012 03:28 AM
Topics I've Started
Directions of genes expression illustrations
12 November 2012 - 06:40 PM
Dear All;
I just Wanna Help from you for a bit confusing problem that I have, I though you all are the best persons to get answer.
Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid.
1.) Why in different plasmids their some genes expressing in different directions? how it happens?
2.) In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
3.) why in same cassette different directions are shown?
4.) In plasmids (e.g. TDNA of Agrobacterium) some of genes marked with <-----> , >------> symbols { same symbols show in transposons too ( <------> )}
Could you please tell me wht tells from these arrow heads?
I've attached some of related figures that I've seen and shown numbers according to questions.
1.), 2.) Q.jpeg 11.4K
71 downloads
3.)Binary vectors used for plant transformation..jpg 35.66K
64 downloads
4.).gif 14.52K
69 downloads
4.).jpg 141.86K
63 downloads
I just Wanna Help from you for a bit confusing problem that I have, I though you all are the best persons to get answer.
Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid.
1.) Why in different plasmids their some genes expressing in different directions? how it happens?
2.) In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
3.) why in same cassette different directions are shown?
4.) In plasmids (e.g. TDNA of Agrobacterium) some of genes marked with <-----> , >------> symbols { same symbols show in transposons too ( <------> )}
Could you please tell me wht tells from these arrow heads?
I've attached some of related figures that I've seen and shown numbers according to questions.
1.), 2.) Q.jpeg 11.4K
71 downloads
3.)Binary vectors used for plant transformation..jpg 35.66K
64 downloads
4.).gif 14.52K
69 downloads
4.).jpg 141.86K
63 downloads
