Akila Wijerathna Yapa
Member Since 12 Mar 2012Offline Last Active Dec 26 2012 03:28 AM
Find content
Male
Posts I've Made
In Topic: Directions of genes expression illustrations
14 November 2012 - 08:01 PM
Trof, on 14 November 2012 - 03:40 PM, said:
1) Why not? Everyones genes are in different directions, why should plasmids be exception? Some viruses even have the same sequence once read from one direction to make one protein and in the oposite direction to make a different one.
3) Not sure what are you refering to, but since it really doesn't matter in which direction a complete casette is (unless in special cases, when some ORF inside other gene can cause blockage of transcription) and usually plasmids were constructed by restriction and ligation, sometimes the ligation is not direction specific so two possible orientations can be created.
4) Arrows usually denote the orientation of repetitive sequences. That is important, sice the repeat direction can have different functions (tandem versus oposite).
3) Not sure what are you refering to, but since it really doesn't matter in which direction a complete casette is (unless in special cases, when some ORF inside other gene can cause blockage of transcription) and usually plasmids were constructed by restriction and ligation, sometimes the ligation is not direction specific so two possible orientations can be created.
4) Arrows usually denote the orientation of repetitive sequences. That is important, sice the repeat direction can have different functions (tandem versus oposite).
Dear Trof; Thank you very much for your kind reply
In Topic: Directions of genes expression illustrations
14 November 2012 - 08:00 PM
ascacioc, on 14 November 2012 - 02:37 PM, said:
1 - you have a promoter before your gene; the RNA polymerase recognizes the promoter and then it goes in the direction of the gene; different gene directions means that the gene is either on the upper or bottom strand; when the RNA polymerase binds to the promoter, it chooses which one is the coding strand of the two possibilities
2 - Both Tet and Amp genes have their own promoters (see above for direction) and terminators. Tet stops before the origin since there is a terminator before the origin starts; hence, there is no collision
Andreea
2 - Both Tet and Amp genes have their own promoters (see above for direction) and terminators. Tet stops before the origin since there is a terminator before the origin starts; hence, there is no collision
Andreea
In Topic: different orientation or direction of genes in expression Plasmid vector
11 November 2012 - 04:15 AM
Julio-Claudian, on 10 November 2012 - 08:03 PM, said:
Dear Julio-Claudian;
Thank you very much for your kind replyn
