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  2. → Viewing Profile: Posts: Sotirios

Sotirios

Member Since 11 Mar 2012
Offline Last Active Apr 26 2013 02:19 AM
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Posts I've Made

In Topic: Help needed! ELISA reproducibility issues?

28 January 2013 - 12:37 AM

1) Check the coating step. Maybe there is edge effect during this step and the problem is in plate, about reproducibility.
2) Check the room humidity. Many times there is a problem with the humidity existance that cause issues when there is delay during the TMB loading step.

In Topic: Green-dye in HRP-Conjugate solution

31 October 2012 - 12:54 AM

Indeed. The green dye is simply there as a visual to aid the loading of HRP-Conjugate solution. The green dye (probably food dye) must not affect HRP function.

In Topic: Green-dye in HRP-Conjugate solution

28 October 2012 - 01:23 AM

for example from abcam elisa kit

In Topic: Competitive vs. Sandwich ELISA

25 September 2012 - 12:36 AM

A competitive immunoassay is more sensitive to matrix issues. In contrast to sandich, in copetitive elisa the matrix effect contributes to the increasment of sensitivity during the competition step.

In Topic: ELISA quantification

07 September 2012 - 01:11 AM

Unfortunately you have to use a program like graph pad or origin

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