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Within the siRNA/miRNA duplex, the guide strand should be the antisense strand to be complementary to the target mRNA (while the passenger strand is 'degraded'). But literature present evidence that it is the terminal nucleotides, its thermodynamic properties, destination argonaute protein, plant vs animal all dictate which strand will get loaded onto the RISC. If the sense strand gets loaded, then the target mRNA will be different. Can both strands potentially induce gene silencing???
#130553 Guide vs passenger strand sorting
Posted
on 06 March 2012 - 06:40 PM
