Sorry if this is an obvious question but Ive just been confusing myself!
I want to make a standard curve for S. aureus to run on my qPCR. I have bacterial suspensions - say 10^8 / ml.
If I just do a bead-beat and lyse the cells, proteinase K treat then etc, then I have a suspension that I can say is 10^8.
But then if I add 10ul to a qPCR, im only actually adding 10^6 gene copies, right? So if I want to do it this way, I need to start at a much higher dilution?
Is this right or am I totally off the mark?
Then, if I get 10ul of sample come up at 10^6, does that mean I have 10^6 in my 10ul of sample!?
thanks so much
firefly2280Member Since 05 Mar 2012
Offline Last Active Jan 19 2013 10:39 AM
- Group Active Members
- Active Posts 6
- Profile Views 551
- Member Title member
- Age Age Unknown
- Birthday Birthday Unknown
My research interests
molecular biology, environmental sampling, POC diagnostics, Viral diseases, ebola etc..!