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srimadh

Member Since 03 Mar 2012
Offline Last Active Apr 12 2013 08:15 AM

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India
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Portraits and still life Painting, surfing thru the literature

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Immunogenetics

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mdfenko
08 Apr 2013 - 04:47
pito
06 Mar 2012 - 09:46

Topics I've Started

Help required - Molecular Concept Maps

07 April 2013 - 07:46 PM

Hi All,

I am actually working on the concept maps of an inflammatory disease ... I wanted to use private.molecularconceptmaps.org website for this purpose. But, I had problems in registering for the website.

It may be weird to ask ... but

Its a request to all, to help me by giving the username and password for this website (private.molecularconcepts.org/).
This is really required for me to check the gene associations to the inflammatory disease and so that I can generate a network.
I will use that purely for my research work and judiciously.

Thanks in advance

With Regards
Srimadh

Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood

27 February 2013 - 12:32 AM

Hi All,

I want to do DNA isolation form whole blood and I need to make these buffers ... TKM1 and TKM2

Can anyone help me with the recipe for the TKM1 and TKM2 buffers.

Thank you

Acetaldehyde estimation in alcoholics

04 February 2013 - 01:02 AM

Hi all,

Can any one tell me a protocol to estimate the acetaldehyde in the blood of people consuming alcohol.

And, in normal or healthy individuals (normal values) what can be the amount and in alcoholics what can be the estimate.

Thanks in advance.

- srimadh

Suggest best protocol for Adenosine deaminase activity

02 December 2012 - 03:04 AM

Hi all!

Can any one suggest me the best method to estimate the activity of adenosine deaminase in the blood.

How we can measure its activity on adenosine ... one way is to know the amount of ammonia being produced,,,

one of the articles I referred did a spec at 630 nm and quantified the ADA activity using Bertholet Method.

But, it did not work good for me ... I got all fluctuated results.. I took care of GLP ... but, I am helpless.

What can be a Perfect Control sample for a Rheumatoid arthritis study?

20 August 2012 - 09:14 PM

Dear All,

I am doing a retrospective study on Rheumatoid arthritis ... say screening the databases for specific tests like RA factor, CRP and ESR, etc. But, to compare with the conrols ... what should be perfect control for the RA study?

Can it be a sample without the symptoms ( according to ACR ) or can it be any other disease other than RA in the whole gamut of rheumatic diseases. I know that the control should be age matched, gender matched, and also ethnicity matched. But, which group of the patients or people can be taken as controls?

Please help me with this ...

Thank you.

With Regards
Srimadh

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