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What types of Taq polymerase do you know if you're saying "which Taq"?
#130838 Real Time PCR polymerase enzyme
Posted
on 12 March 2012 - 04:41 AM
#130842 Real Time PCR polymerase enzyme
Posted
on 12 March 2012 - 04:59 AM
At least, it should have a 5' to 3' exonuclease activity for TaqMan assays. 
The lower temperature will affect the TaqPol, the velocity of extension will be decreased (this is one reason why you should keep real-time PCR products short).
The lower temperature will affect the TaqPol, the velocity of extension will be decreased (this is one reason why you should keep real-time PCR products short).
