Hi drwho and thanks for your reply. we already did a dose response curve for 3 days using different IGF1 concentrations (750ng/ml; 500ng/ml; 250ng/ml; 125ng..62,5..32...16....8ng/ml) and found increased RNA expression of our marker genes until 125ng/ml (marker genes were: PHLDA, CSF1, VEGFA- normalized against 3 housekeeping genes, which were all stable). then signals decreased again (thus no sigmoid curve but an inverted U). we are still wondering, whether this is due to apoptotic effects (but I cant find any LD50 for IGF1) or by differentiation of the moncytes into macrophages.
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