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I always used to add antimitotics while differentiating these cells. Also RA after a while storing at -80C can degrade, so i get new ones frequently.
We use Neurobasal with B27 as the differentiating media and no FCS.
#128887 RA-differentiation of SH-SY5Y
Posted
on 10 February 2012 - 11:09 AM
