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Smog187

Member Since 09 Feb 2012
Offline Last Active Mar 04 2013 06:53 PM

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#141601 Method for protein concentration measurement

Posted Smog187 on 16 September 2012 - 04:32 PM

Rchqing, on 14 September 2012 - 09:39 PM, said:

Smog187, on 13 September 2012 - 06:32 PM, said:

If you know the amino acid sequence then I would trust the Native scan. You could determine the concentration by denaturing your protein with some NaOH and using the extinction coefficient of your protein with the Tyrosines and Tryptophans at 260nm (if you have these amino acids).

Thank you very much! Could you give some suggestions about how much NaOH is needed?

We use a final NaOH concentration of 0.1N in the cuvette. Also, make sure the cuvette you're using is designed to be used in the UV range.