Thanks for the replies: When I did the colony PCR I used the same primers I used for the INfushion reaction, so "hopefully" the only product being made is the insert.
You could still do the BCA protein quantification and run in parallel a known set of concentrations and then compare the color you get to your samples to get a "rough" estimation of protein concentration. Or you could run a SDS gel and load with known protein concentrations and compare your samples.
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