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Maya1988

Member Since 30 Jan 2012
Offline Last Active Dec 11 2012 07:11 AM

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Group Active Members
Active Posts 9
Profile Views 828
Member Title member
Age 25 years old
Birthday February 5, 1988
Gender
Female
Location
United Kingdom
Hobbies
Modern, Cool Nerd

83 % Nerd, 52% Geek, 22% Dork

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My research interests
Biochemistry

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Latest Visitors

uday
28 Nov 2012 - 18:18
invasifspecies
20 Aug 2012 - 09:22
Curtis
12 Jul 2012 - 06:24

Posts I've Made

In Topic: In situ hybridization - huge background on slide where the paraffin was?!

27 November 2012 - 06:15 AM

Dear bob1,

Thank you for getting back to me. At which step do you suggest doing this wash? I am doing fixation overnight when I collect the tissue, and then there are two more fixation steps in my protocol (already after the deparaffinization steps).

Best,
Maya

In Topic: IGF2 antibody for immunohistochemistry/WB in mouse?

14 November 2012 - 01:55 AM

Dear Curtis,

Thanks, I did that on the same day as I posted this question. Also got some replies and will try the one they have! Hopefully it works...I'll report back!

In Topic: Welcome new Bioforumer, Tell us a little bit about yourself

11 July 2012 - 07:25 AM

Hi all *waves*,

I am Maya and I am a 1st year PhD at Cambridge. My first year report submission deadline is just ahead...I love research but I also hate it. I guess it's a bit like with Gollum and the ring, lol.

Anyway, I am quite new to the forum and thought I'd introduce myself...

Have a nice day,
M =¬.¬=

In Topic: ISH - signal in sense - specificity or contamination problem?

11 July 2012 - 05:55 AM

Hi alexa594,

I have not left the liver S overnight so I can't be 100% sure that I wouldn't have ended up with some background there, too. However, none of the slides which I left incubated overnight dried out (at least not obviously so) - when I stopped incubation the next morning, all of the sections were still completely wet (I circle with pap-pen and actually add 400ul of hyb buffer with probe to each section). So I am quite sure this is not simply unspecific background due to drying out of sections, especially since both AS and S seem to selectively stain specific cell types only within the tissue of interest.

In Topic: Why in Situ in rat works but not in mouse

10 July 2012 - 03:17 AM

Hi Montys,

I am not sure if you are still waiting for an answer to your question. I am doing ISH and I am also experiencing some problems. Did you check that the probe you are using is specific to mouse? If it is a probe designed for a rat gene, that might be the reason it doesn't work in mouse?

Best,
Maya

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