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mbgene

Member Since 22 Jan 2012
Offline Last Active Nov 26 2012 08:54 AM
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Topics I've Started

SV40 promoter

26 November 2012 - 08:54 AM

Hello,

Can anybody tell me how constitutive the SV40 promoter is? Compared with CMV promoter, is the SV40 promoter active in as many different cell types as the CMV promoter?

Does anybody have experience with whether SV40 promoter is active in human primary cells such as bone marrow stromal cells?

Thanks a lot!

Fibronectin coating

17 September 2012 - 12:13 PM

Hello everyone,

I have a general question regarding coating tissue culture plates to culture cells on.

If you are coating plates to culture human cells, is it standard to used human-derived coating solutions (e.g. fibronectin), or would coatings derived from another species also work?

Thanks a lot in advance for your help!

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