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Tabaluga

Member Since 20 Jan 2012
Offline Last Active Today, 01:42 AM

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Member Title Hakuna Matata
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Posts I've Made

In Topic: Literature quote guessing game

Yesterday, 02:24 PM

Bambi, a life in the woods by Salten ? Author could fit somehow (had to emigrate, Zurich, died soon after)

In Topic: CO2 to sedate animals

Yesterday, 01:57 PM

Interesting discussion. In our lab the mice to be killed are put into a CO2 chamber. Then they get convulsions and you can observe a really heavy breathing. Posted Image

They are then taken out and killed by breaking the neck. I always wondered at which point they are dead ? At the moment of breaking the neck or already before ? Can someone tell me ?

For sedation they get something injected, but I don't know what.

In Topic: How to culture HK-2 cells

Yesterday, 01:49 PM

About the 'how to prepare': When you know the ingredients of your medium (like from the tab "Culture method" of the link above), you just mix them together and take note of possible extra instructions (like the one from that website, that the medium shouldn't be filtered).
Also, the described medium contains EGF, so I think the medium should not be warmed quickly in a waterbath but acclimatise at room temperature instead (At least our lab always handles it like that when a medium contains growth factors, please correct me if you think this is not standard/not necessary ?!)