Hi guys I have read in other forums that this is the best way?
Sip up whole media(wit unevenly distributed cells in the well) in to a tip n release them again 2 to 3 times. it wrked for me.for larger plates up n down left n rite rocking for 3 times is enuf.
Also -you put your cell suspension in the well,
-pipette a little bit,
-then put the lid on the plate and
-draw an "eight" with your plate as a "pencil" and the hood as a "notebook"
Also rocking back and fourth/side to side, then do the figure eight technique with the plate, and lastly let the plate sit for about 40 minutes in the hood before I proceed to move the plate into the incubator.
Does anybody have have any preferences?
shamash119Member Since 18 Jan 2012
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19 Jan 2012 - 05:26