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Further note that p53 expression is rather transient and quite quick (approx 8 hours post insult). I have found that the best/easiest way to induce p53 is by irradiating with UV at 10 mJ/cm2, and then harvesting between 6 and 10 hours post irradiation.
#127159 U2OS treatment with etoposide
Posted
on 16 January 2012 - 12:14 PM
