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Dear
jerryshelly1
Thanks for helpful advice
Madeline
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In Topic: Quantification of Fluorescent images
17 February 2013 - 03:17 PM
In Topic: Centrifugation speeds for cells.
17 February 2013 - 03:16 PM
Dear Guys,
I know this is out of ur discussion, but regarding converting RPM into G or vice verse, I just measure the radius of my centrifugal ring, not the whole machine, just the rotor radius with a ruler or something, put the data on RPM-G converter like these website:
http://www.endmemo.com/bio/grpm.php
http://www.geneinfin...p/sp_rotor.html
then I got the required number in G or in RPM
I know this is out of ur discussion, but regarding converting RPM into G or vice verse, I just measure the radius of my centrifugal ring, not the whole machine, just the rotor radius with a ruler or something, put the data on RPM-G converter like these website:
http://www.endmemo.com/bio/grpm.php
http://www.geneinfin...p/sp_rotor.html
then I got the required number in G or in RPM
In Topic: Quantification of Fluorescent images
13 February 2013 - 09:37 PM
Dear
bob1
Thank u for ur advice, I already download this program, but as I have no experience in using it, I wanted some advice, links, PdF or tutorials regarding how to use this program, I have found a couple of tutorials online, but they have not cover all my question yet.
bob1
Thank u for ur advice, I already download this program, but as I have no experience in using it, I wanted some advice, links, PdF or tutorials regarding how to use this program, I have found a couple of tutorials online, but they have not cover all my question yet.
In Topic: Quantification of Fluorescent images
13 February 2013 - 09:35 PM
jerryshelly1
Thank u very much this blog is very helpful,
I have a question for u:
If I want quantify the Reactive oxygen species using Fluorescence agent, I would want to calculate fluorescence inside the whole cell .
My question which is more meaningful, using high magnification images (100X, 40X) or using low magnification (20X).
from my point of view and my pic: low magnification is better, give me a lot of cells in the same pic, besides I can see fluorescence better in low magnfication pic, but not details, as I dont choose fluorescent spot but the whole cells
or it is more meaningful to use high mag pictures and analyze.
Thank u very much this blog is very helpful,
I have a question for u:
If I want quantify the Reactive oxygen species using Fluorescence agent, I would want to calculate fluorescence inside the whole cell .
My question which is more meaningful, using high magnification images (100X, 40X) or using low magnification (20X).
from my point of view and my pic: low magnification is better, give me a lot of cells in the same pic, besides I can see fluorescence better in low magnfication pic, but not details, as I dont choose fluorescent spot but the whole cells
or it is more meaningful to use high mag pictures and analyze.
In Topic: Quick question about making culture media.
08 January 2013 - 06:54 PM
if u did not remove medium before adding FBS, cocn of FBS would be 9 % not 10 %.
as it would be 50 FBS in 550 ml medium volume
for accuracy, it should be the same to repeat ur experiment even after years
I seen people just added volume of FBS/supplement without removing any part of medium.
I guess this is not correct.
as if u would write in ur published work I used 10 % FBS medium it must be 10% not 9%.
I guess what
Myworkismyplay
means
That u remove equivalent volume to ur added constituents in clean sterile container, in the clean bench, then added ur supplements (FBS/Antibiotic), the final volume should be 500 ml, or u must complete it to be 500 ml
this is how I understand her words
as it would be 50 FBS in 550 ml medium volume
for accuracy, it should be the same to repeat ur experiment even after years
I seen people just added volume of FBS/supplement without removing any part of medium.
I guess this is not correct.
as if u would write in ur published work I used 10 % FBS medium it must be 10% not 9%.
I guess what
Myworkismyplay
means
That u remove equivalent volume to ur added constituents in clean sterile container, in the clean bench, then added ur supplements (FBS/Antibiotic), the final volume should be 500 ml, or u must complete it to be 500 ml
this is how I understand her words
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