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  2. → Viewing Profile: Topics: rameshcdri

rameshcdri

Member Since 19 Dec 2011
Offline Last Active Yesterday, 09:49 AM
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Topics I've Started

How to predict the number of binding site (along with sequence of binding site)

30 August 2012 - 11:16 AM

Hi All,

I need one favor. Can you please send me link of any online server. I want to predict the number of binding site (along with sequence of binding site) in mouse genome by my gene of interest.

i.e. my gene of interest is 'A', I want see the number of gene regulated by A by binding of this on promoter or other regulatory sequence.

Please suggest me.

Thanks.


Posted Image
Ramesh

Is the Kozak sequence really needed for cloning to express the gene?

24 July 2012 - 07:14 AM

Hi Friends,


I am cloning my gene of interest in pAAV-DIO vector to cell specific expression in brain. However, my gene doesn't present any kozak consensus sequence upstream and downstream of the ATG naturally. The Kozak consensus sequence plays a major role in the initiation of the translation process. Therefore, I can add kozak sequence upstream of ATG; however it is not possible in downstream. This manipulation might work. Even without the Kozak sequence, the translation efficiency of the protein might drop, or will drop. I do not have experience with the mammalian expression system. Is the Kozak sequence really needed for cloning?

Kindly suggest me.

Thanks,
Ramesh

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