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Thank you all for your help.
I pretend to culture primary pancreatic cells, The problem is that I am starting a suspension culture and doing a first step of attached conditions (which would allow me to wash off the unattached RBC's) may compromise my culture.
I will try the treatment on ice, and take into account that I am selecting for a more resistante population. However, if you think that there is an alternative, please do tell me.
Thank you very much.
BW,
Rute
