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Recently, we have noticed our 1x SDS running buffer becoming 'contaminated'.
It becomes thick, more viscous then our 10x stock or gooey/goopy. After running and transfering our gel, ponceau staining of the membrane shows a high background starting at the dye front and continuing to enter the gel throughout the run as though there is some sort of contaminating protein in the buffer. The 10x stock is fine and 1x is made in a clean glass bottle with autoclaved water and make in 2L batches which is not kept for more than a couple weeks at a time. 1x buffer is not reused.
What could possibly grow in SDS? Another lab that also works in the space has seen a similar problem. Does anyone have any idea what this might be?
#124171 SDS-PAGE Running Buffer contamination
Posted
on 23 November 2011 - 09:21 AM
