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Fugene usually works at a ratio of 3:1 based on microlitres of fugene: ug of DNA, so for your first row you would use 550 ng =0.55 ug x 3 (wells) = 1.65 ug DNA x 3 (from the ratio) = 4.95 ul fugene.
#129804 Fugene6 Calculations aren't working....please advise???
Posted
on 23 February 2012 - 04:09 PM
