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Is image beutification (changing image contrast/brightness/sharpness) the same as image fabrication (e.g. false information, adding or removing pixels, etc)?
Some papers now require authors to attach original images when they submit. So if this is the case, does it mean researchers have to try their best to capture the perfect image (from the microscope, chemiluminescence machine, camera) and not beautify it through post-processing? (Note: post-processing meaning perfecting images but not adding/removing pixels)
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Image beautification?
06 June 2013 - 11:36 PM
Autoclaving water to go into the incubator
30 May 2013 - 09:49 PM
Is it common to autoclave distilled water to be used for humidifying a cell culture incubator? How is the risk of getting contamination from the loosely screwed bottle (during autoclaving)?
Sediments/debris/pellet in protein lysate?
14 March 2013 - 01:06 PM
My total protein was obtained using a very standard protocol of lysing adherent cells, sonicating samples to shear DNA followed by centrifuging them for 15 min to separate DNA/debris from protein. Total protein samples were stored at -20C for approximately 1.5 months before I thawed it out to run my SDS-PAGE and ultimately westerns.
I saw that there is some kind of foreign material in my lysate in about 80% of samples I'm going to run. It looks like DNA residue or some kind of crystallised debris. It doesn't resuspend into the protein lysate but remain as pieces of debris. Anyone had this before? Could it be some kind of contaminate (fungi or yeast)? or Leftover DNA which was not cleared from the spin? It's most likely not DNA since it DNA pellets were well separated from protein suspension after lysis.
I saw that there is some kind of foreign material in my lysate in about 80% of samples I'm going to run. It looks like DNA residue or some kind of crystallised debris. It doesn't resuspend into the protein lysate but remain as pieces of debris. Anyone had this before? Could it be some kind of contaminate (fungi or yeast)? or Leftover DNA which was not cleared from the spin? It's most likely not DNA since it DNA pellets were well separated from protein suspension after lysis.
Non-reproducible results/experiments
14 March 2013 - 03:37 AM
I keep hearing PhD students venting about their inability to repeat experiments and get the same outcome based on the methods provided by published papers from another lab or even protocols produced by someone in the lab (same concentration, same cell lines, same conditions etc). Is this common?
I've even heard people getting strangely opposite results (e.g. published as upregulation but instead we see downregulation)
Then it comes down to whether the published protocols are legitimate or it's purely our own fault when repeating someone else's technique?
I've even heard people getting strangely opposite results (e.g. published as upregulation but instead we see downregulation)
Then it comes down to whether the published protocols are legitimate or it's purely our own fault when repeating someone else's technique?
To design or use published primers?
13 March 2013 - 02:45 AM
Is it better/preferable to design your own sets of primers to a particular gene or use ones which have been published in papers looking at the same gene?
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