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  2. → Viewing Profile: Posts: Neanderthal

Neanderthal

Member Since 15 Nov 2011
Offline Last Active Apr 03 2013 08:32 PM
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Posts I've Made

In Topic: random primers or oligodT

20 March 2013 - 03:39 AM

When you checked your 12 genes of interest on cancer cell lines (whose RNA was extracted using olidodT primers) did you come across any problems? Is that the reason you want to use oligodT? If you have stumbled upon any problems, then why can't you sgo ahead with random hexamer primers? I am curious to know if there is any specific reason.

Kart

In Topic: RNA gel electrophoresis for integrity check - denaturing or non-denaturing??

12 March 2013 - 09:06 PM

Thanks Trot for the link. It is really helpful.Posted Image

In Topic: Optimizing reactions for qRT-PCR using regular thermocycler

11 March 2013 - 11:15 PM

Thanks alot Wasabi and Trof for your inputs. I'm using SYBR by the way. If I understood it right, regular PCR will only give if there is a nonspecific binding or not, and since the same master mix as qPCR should be used, there won't be any help with savingreagents also.

In Topic: RNA gel electrophoresis for integrity check - denaturing or non-denaturing??

11 March 2013 - 11:08 PM

oh..yes, I meant loading dye. Thanks a lot for the links.

In Topic: RNA gel electrophoresis for integrity check - denaturing or non-denaturing??

07 March 2013 - 11:37 PM

If I can use TAE buffer that I use for DNA, can I use the same loading buffer also?

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