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I am designing a project involving non-tumor DNA. We intend to do whole genome sequencing. So I was wondering what would be the sample of choice? Buccal swab? Saliva? Chewing gum? Mouthwash?
Do I get enough material for next generation sequencing from these?
What are the considerations (smokers)?
Which are the pros and cons of there different methods (swab vs. saliva vs. other)?
Since I have no experience with NGS and isolation of DNA from such tissues any help/advise would be appreciated!!
)All the best,
the rat
