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Using trypsin before FACS is not a good idea. Trypsin cuts at any site following Arg and Lys in an indiscriminate fashion. So there is a strong possibility of losing cell-surface marker which result in decreased FACS efficiency.
We use non-enzymatic celll dissociation solution to dislodge cells from adherent-monolayer cell-culture.
#119996 FACS Sorting and Protein Purification
Posted
on 19 September 2011 - 08:35 PM
