Using trypsin before FACS is not a good idea. Trypsin cuts at any site following Arg and Lys in an indiscriminate fashion. So there is a strong possibility of losing cell-surface marker which result in decreased FACS efficiency.
We use non-enzymatic celll dissociation solution to dislodge cells from adherent-monolayer cell-culture.
jarannihcMember Since 29 Oct 2011
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Immunology, pregnancy,molecular biology,macrophages, natural killer cells, surfactant proteins