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Cts of 34-35 are already quite high. Do you know if this Ct range is still in your linear dynamic range of the assay? If not, you cannot quantify it. How did your non-template control look like, because your are also getting in your NC a signal in one replicate?
#122199 qPCR result interpretation - when your 'relative' is 0??
Posted
on 23 October 2011 - 11:48 PM
