I really appreciate your useful tips and encouragement. Another interesting thing is I feel I can do better presenting others' work in journal club than presenting my own research. I guess that is how "telling a story" makes a difference. When I do journal club, I can "tell" a story, but not so good at telling my own story.
Yes, I agree. Behind those pretty story, there are always lots of trials and errors, and frustration. But eventually they got there. So I guess I have to keep trying.
Thank you for the helpful information. Do you use any kind of enzyme to dissociate the cells besdies mechanic method? Can you store the tissue or cells for late analysis on FACS machine?