Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

joy123

Member Since 21 Oct 2011
Offline Last Active May 02 2013 09:48 AM

Find content

Community Stats

Group Active Members
Active Posts 102
Profile Views 1,045
Member Title Veteran
Age Age Unknown
Birthday Birthday Unknown
Gender
Not Telling

Contact Information

2 Neutral

Friends

joy123 hasn't added any friends yet.

Latest Visitors

bioforum
20 Sep 2012 - 14:15
pDNA
29 Feb 2012 - 10:58
merinator
28 Oct 2011 - 15:51
macbookairuser
28 Oct 2011 - 15:48

Posts I've Made

In Topic: urgent: need help with qPCR statistics

26 April 2013 - 09:37 AM

Thanks for your reply! Unfortunately, my sample is a mixture of human cells and bacteria, and the cell munbers vary among people. So I don't have internal control. I loaded same amount of DNA (which has uncertain amount of human and bateria DNA) in the qPCR analysis, and compared the level of 16S gene to compare the amount of my interested bacteria in same amount of samples of different groups of people.

In Topic: acceptable melt curve?

14 February 2013 - 08:36 AM

Hi! Can anybody please help me with this problem? Thanks!

In Topic: good 16S univ. primer for qPCR?

30 January 2013 - 10:11 AM

Thanks! Do you mean search "16S" in the "QIAGEN Reference Database"? That comes only 3 articles that using their reagents. Do you mean Qiagen website have other better way to look for primers? I appreciate your help!

Curtis, on 29 January 2013 - 08:33 PM, said:

check Qiagen website, you need shorter amplicons, below 150 bp is desirable.

In Topic: isoelctric gels hard to stain?

26 January 2013 - 08:10 AM

Thanks for your response!
The background was very faint after destaining.
Indeed, the articles used more sample than I did. I think maybe silver staining would be a good try.

In Topic: DNA lost dramatically during storage

21 September 2012 - 06:12 AM

phage434, on 20 September 2012 - 04:44 PM, said:

I'd strongly recommend storing your DNA in TE rather than in Tris buffer.

I read sometines EDTA interfere with PCR assay. I wonder if it is safe to use TE for qPCR? Thanks!