Hi! I'm currently doing co-transfection of my target plasmid and pSEAP control plasmid (Clontech) using Xtreme-gene HP from Roche. I would like to know how to calculate the transfection efficiency through SEAP reporter gene assay. Thank you!
Hi, everyone! I recently encounter a problem in SDS-PAGE for mammallian cell culture medium.
I was doing a transient expression of a target protein (~16kDa) in CHO cell using DMEM+ 10% FBS medium. I collected the culture medium and load 10μl onto 4%-12% Tris-Glycine gel together with Novex Protein Sharp Prestained Standard. During the electrophoresis, I observed that the last three bands of ladder (15kDa, 10kDa and 3 kDa) were packed in a 3mm clear zone across the whole gel. And after staining, there is a big bulk of albumin.
What can i do to remove this bulk of albumin and concentrate my samples without using any kits?
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