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Hello,
I have not much experience with LC480 but recently Iīve tried some duplex Real-time PCR on LC480. Itīs a duplex reaction using FAM and HEX probes (using Color Compensation). After 2nd Derivative Max Analyses amplification curves of some samples (they should be negative) do not show any amplification. However, the software gives some Cp in the table. Could somebody advise me how is that possible and I could improve the data?
Thanks a lot.
Hana
