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Dimers tend to form when the template concentration is low. If you can, try titrating in more of the template.
Adding yeast tRNA carrier can reduce the amount of template that sticks to the tube walls, making more of it available for amplification.
When designing your primers, check them for homo-dimer and hetero-dimer formation with the Oligo Analyzer program at the IDT website www.idtdna.com.
Soumyajyoti Chowdhury
Member Since 09 Oct 2011Offline Last Active Jan 11 2012 03:27 AM
#8198 How to get rid of PCR primer dimer
Posted
on 26 October 2004 - 03:45 PM
