Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account
  2. → Viewing Profile: Topics: Afiqah Alyaa

Afiqah Alyaa

Member Since 04 Oct 2011
Offline Last Active May 13 2013 05:25 PM
-----

Topics I've Started

Which is a better way to culture cells after thawing ?

14 September 2012 - 03:35 AM

Centrifuge the thawed cells in culture medium and resuspend the pellet then only transfer it to a culture flask
OR straight away culture the thawed cells from the cryovial in a culture flask ?

Difficulty in cutting tissue after collection of sample in RNAlater

14 September 2012 - 01:29 AM

I kept my tissue samples in RNAlater after collection from the OT.
The samples were kept overnight in 4 degrees, and transferred to -80 degrees the next day.

When trying to cut the tissue samples with cryostat,
the samples seem to split and break and I cannot prepare my slides with this samples.
Before subjecting my samples to the OCT medium, I just dab the samples on a tissue paper to get rid of the RNAlater.

So do the difficulty in cutting the tissue samples are caused by RNAlater ?
How to remove the RNAlater covering the tissue sample effectively before subjecting them for slide preparation ?

Hope that someone out there can help me to overcome this problem.

Thank you.

Relative fold expression and Normalization fold expression

06 July 2012 - 03:11 AM

Hi !

I have a question regarding the analyzation of qpcr data.
How do you calculate relative fold expression and normalization fold expression ?
My samples would be tumor versus normal cells.
The tumor and normal cells were taken from different patients.

Thank you.

  1. BioForum
  2. → Viewing Profile: Topics: Afiqah Alyaa
  3. Privacy Policy
Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.