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Anula

Member Since 02 Oct 2011
Offline Last Active Jan 31 2013 03:39 AM
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Topics I've Started

survival strategy of bacteria

11 January 2013 - 04:48 AM

Hi Everybody!

Do You know why some species of bacteria survive in macrophages? I need to find something about their strategy!!

Anula

Flow cytometry with one fluorochrome

21 June 2012 - 10:47 PM

Hi everybody!!

I'm starting work with flow cytometer (FACS Canto II) and I have some problems. I try to detect caspase 3 in macrophages upon camptothecin and E. coli contact. I use unstain non-stimulate control, FITC-conjugated non-stimulate control and my samples. Do I have to do compensation If I have one fluorochrome??  And which population should I gate??? Please give me some advice..

Anula

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