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  2. → Viewing Profile: Posts: Anula

Anula

Member Since 02 Oct 2011
Offline Last Active Jan 31 2013 03:39 AM
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Posts I've Made

In Topic: survival strategy of bacteria

14 January 2013 - 04:14 AM

Thank You very much!

Epecially I am interested in survival strategy of E. coli. I know that these bacteria produce catalases which destroy H2O2.
But what else?

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In Topic: Flow cytometry with one fluorochrome

27 July 2012 - 10:05 AM

Hi!

I'm dealing with THP-1 (human monocytic cell line) that I differentiate to macrophages. So if I well understood I should use as a control only macrophages without contact with E. coli??? What about some positive control???

Anula

In Topic: Differentiating Thp-1 into macrophage

27 July 2012 - 04:22 AM

Hi PHVS!

I've read a lot of papers about PMA and finally I decided to do fresh medium every time I need to. Everything works well:)

Macrophages are mature form of monocytes and they don't proliferate after differentiated.

Anula

In Topic: Flow cytometry with one fluorochrome

27 July 2012 - 04:18 AM

Hi!

I'm a PhD student and I'm going to examine expression of PAR2 receptor on macrophages upon E. coli contact. I am a new one in Flow cytometry and I am not sure for example what controls I should include in my experiment....

Thanks for any advice:)
Have a nice day
Anula

In Topic: Flow cytometry with one fluorochrome

24 July 2012 - 04:02 AM

Thanks a lot for Your reply:)

Have You ever examined expression of receptor by using FC?

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