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>reverse complement TTACCAGCGGGGTGTGATATGACCTAAGGTCCCGAACTCCTTCGAGAATACTTGGCTTAC TGGAAAGAAGGCGCATCATAGACACCACTTCATCAGGGTAGTGTGTCTATATGTGTTGCC TTATTACCAGAAGCATAAAAGCAGCATTTAGACCTGGTCATAAAGCCCCTGAGATGATTA GCCTTATAGTCAGCTTTTTTACTGTAGCCATGTGTAGCTAAGGCCCCCTCCCTTTTCCAG ACACAGAAGACACAAACAAAGAACTTCAGATTTTAACACA
I assume you know that you have to incorporate restriction site to the 5' end of your PCR primers so that the imRNA can be cloned into an expression vector from 5' to 3' end.
Actually you don't even need to reverse complement your sequence, you can still use the original sequence to design primers and clone the miRNA in the right orientation by controlling which restriction site goes to which primer.
