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Hi friends
I have pBluescript vector and e coli which I made by myself. I used Onetaq (NEB) for my pcr reaction. I used degenerate primers and I dont now full sequens of my sample, neither enzym cut site.
My questions
1. should I do any treatment to my pcr product before ligation
2. I dont know enzym cut site of my sample. If I use EcoRV for blunting end. Does it work.
3. If I directly ligate my pcr product to vector. what happens. Does it work
Thanks
