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  2. → Viewing Profile: Posts: paulis

paulis

Member Since 14 Aug 2011
Offline Last Active Nov 21 2012 07:20 AM
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Posts I've Made

In Topic: Luciferase transfection controls

21 November 2012 - 07:20 AM

Hi everyone,

I'm doing a Renilla luciferase assay to measure the activity of protein promoter "A", and I'm transfecting with another plasmid, which is the protein "A" fused to GFP, so my question is, if I have some measures problems when measuring luciferase activity with luminometer, I mean GFP could interfere with the measurement of luciferase?

Please help me Posted Image

In Topic: GFP and luciferase measurements

21 November 2012 - 07:15 AM

Hi everyone,

I'm doing a Renilla luciferase assay to measure the activity of protein promoter "A", and I'm transfecting with another plasmid, which is the protein "A" fused to GFP, so my question is, if I have some measures problems when measuring luciferase activity with luminometer, I mean GFP could interfere with the measurement of luciferase?

Please help me Posted Image

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