Because the dye (e.g. EB) moves the opposite way from your DNA, that is why the bands at the bottom are not visible anymore. There are ways of walking around: 1) don't use the last row of wells, or 2) after running stain the gel with EB; or 3) add EB to your buffer.
Papaver
Member Since 10 Aug 2011Offline Last Active Sep 14 2012 03:59 AM





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