Find content
Not Telling
yes it can ...there should be no interference (not to my knowledge)!
Regards,
p
#122570 Cloning a promoter
Posted
on 28 October 2011 - 10:00 AM
#122110 Cloning two fragments into a vector
Posted
on 21 October 2011 - 05:48 PM
3. prepare vector, A, and B as above and do a triple ligation reaction. If you have good vector and A and B DNA samples and properly cut ends, then triple ligation is quite easy. I'd recommend cloning into a vector with different antibiotic resistance than the ones containing A and B. You can reduce vector only background by using PCR to amplify your vector as well, and then digesting with DpnI to remove undigested template DNA. If you do this with enzymes that can be heat killed, you don't even need to purify your digestion products.
