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sultan

Member Since 28 Jul 2011
Offline Last Active Apr 29 2013 02:10 AM
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Topics I've Started

How to Join two PCR products

27 November 2012 - 06:59 PM

Hi
I need to join two PCR products in to a single one. I have heard about fusion PCR but I have never used it before. Any body has any idea about how to design primers to fuse two PCR products?
Thanking in anticipation.

How to join two sequencing files taken form forward and reverse primer

17 November 2012 - 11:42 PM

Hello to all,
               I am new to sequencing field. I have a gene that is inserted in PCR 3.1 vector between T7 promoter and BGH terminator. My gene size is 1.8 kb. I have sequenced the vector with T7 promoter forward primer and with BGH reverse primer (both have given more than 1000 quality sequence read). I have got two sequencing files. Can any body help me that how can I get join the the results from both forward and reverse primer to get the complete sequence of the gene.
Thanking in anticipation.

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