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witioni

Member Since 26 Jul 2011
Offline Last Active Aug 28 2012 02:22 PM
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Topics I've Started

Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl

28 August 2012 - 01:54 PM

Going to create an 18s pr/pb set for use with a human, rhesus, and cynomolgus assay.

Found a set from literature but not too sure if it will pick up rhesus and cynomolgus. Nothing shows on blast.

Cannot use ABI set because we want to modify the primer set a bit and, of course, ABI will not release the sequence.

Will this primer probe set developed for human 18s work on Rhesus and Cynomolgus? I would believe so considering the gene is evolutionarily slow to change and the close relationship between all three species.

Thoughts?

Thanks in advance!

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