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  2. → Viewing Profile: Posts: srpres

srpres

Member Since 18 Jul 2011
Offline Last Active Mar 12 2013 12:00 PM
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Posts I've Made

In Topic: polymerase to use for cloning

06 March 2013 - 12:43 PM

View Postbob1, on 06 March 2013 - 11:47 AM, said:

I like KOD, but the pfu range tends to be the standard.
What is KOD? What do you mean by "pfu range?" Thanks.

In Topic: Phenol ChCl3--upper or lower phase (?)

22 October 2012 - 12:40 PM

I thought that the upper phase was some kind of buffer as well. However, I looked at the MSDS and it said that there was phenol, ChCl3, and IAA. It did not mention anything else, like Tris. So I was suspicious the upper phase was something useful to the extraction.

In Topic: problem with pGL3basic luc activity

11 September 2012 - 05:15 AM

pGL3 has relatively high background activity which can be inducible upon stimulation with exogenous agents. I think its because of some kind of cryptic promoter activity. I know that pXPG-Luc has lower background activity (like 20 x lower than pGL3 Basic in HEK293 T) but also I strongly suspect the new version pGL4 Basic is better as well. pXPG is avaialble from Peter Cockerill and pGL4 from Promega.

Here is a useful reference:

http://www.ncbi.nlm....=pXPG Cockerill

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