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ACN

Member Since 12 Jul 2011
Offline Last Active Mar 21 2013 11:06 AM

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Group Active Members
Active Posts 17
Profile Views 3,053
Member Title member
Age 26 years old
Birthday October 4, 1986
Gender
Male
Location
Irvine, CA

About me

My research interests
Virology, evolution, infectious diseases

Contact Information

Website URL http://andren32@gmail.com

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Posts I've Made

In Topic: Best way to isolate viral mRNA for RT-PCR?

26 February 2013 - 08:50 PM

Yeah, you're making a lot of sense! I all ready have the specific viral primers, used for the RT-PCR, but I am not sure if they are too close to the host cells. I actually never questioned why I can't just use total RNA, I'll bring it up to my P.I. tomorrow for sure.
Thanks for the help!

In Topic: Best way to isolate viral mRNA for RT-PCR?

26 February 2013 - 07:55 PM

Thanks for the info! Will this work for specifically isolating mRNAs and not total RNA ?

In Topic: Quantifying Viral mRNAs at Different Time Points?

27 November 2012 - 11:11 AM

Okay cool! Thanks for the heads up!
Guess I'll just stick to the Real-Time Quantitative RT-PCR.

In Topic: secondary antibodies!

16 October 2012 - 07:13 AM

casandra, on 15 October 2012 - 01:07 PM, said:

This: http://www.piercenet...e-second-Ab.pdf will give you a good overview. We usually get our labelled secondaries from Jackson Immunoresearch...they are reliable and the prices are quite reasonable.

Thanks, that link didn't seem to work for me; but, I'm finding some really useful information here: http://www.pierce-an...ary-antibodies/

In Topic: Chicken Egg Virus Harvest (Yolk Problem)

17 September 2012 - 09:31 AM

We infect the chicken eggs when they are 10 days old. Yolk is in the samples because, I must have ruptured the yolk membrane, allowing the yolk to spill out into the allantoic/amniotic fluids. The yolk is not greenish and my PI did not think there was a contamination. Only a few of our samples have yolk in them. I am not sure of the virus titer; however, the virus stocks that were used for infection, were chosen by our lab’s PI (Therefore they should have been appropriately selected).

If you have a digital protocol of your egg infection/virus harvest, I could I bother you to email it to me. I’d like to review what your lab does differently than ours.

Thanks!

-ACN

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