I think so..But did you find any solution for this issue? Since I design the peptid structure do you think I should change some part of it? I heard somewhere that it might be because of ribosom machinery. It synthesizes the peptide insufficiently and stop some where.
Actually I think they shouldn't be non specific bands, because they are expressed after IPTG induction and they can be seen even after Ni-NTA chromatography(His-Tag). I need a pure recombinant peptide but I don't know why I see multi bands at every steps!
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