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  2. → Viewing Profile: Posts: @bhijit

@bhijit

Member Since 23 Jun 2011
Offline Last Active Mar 14 2013 04:12 PM
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Posts I've Made

In Topic: Problem amplifying viral gene

06 March 2013 - 07:40 AM

I am talking about porcine parvovirus. I am going to use normal taq as suggested above


Also i read that the rna formed from this virus undergoes alternate splicing could it affect my pcr in anyway ??

In Topic: Viral genomic DNA isolation ?

22 November 2012 - 06:19 AM

Pardon me - but I am new to this field

Yes the virus will be from tissue culture. To recover virus [lyse the tissue culture cells and thus virus will be in supernatent] my friend suggested me to use - freeze thaw.

But he also showed me another protocol without any cell lysis. so I am confused what I should use ?

Yes as leelee suggested - PCR doesnt need pure DNA - if there are any other methods please suggest

I want to sequence this PCR before cloning so I am thinking if the DNA is pure it would be better in PCR.

In Topic: How to determinate concentration of conjugated Ab`s ?

24 October 2012 - 06:46 PM

using the common protein estimation methods should work

In Topic: Mouse IgG detection - Blocking ?

24 October 2012 - 06:44 PM

To add - even control mouse will non-specific Ab in blood - How can I minimize the reaction with these antibodies. Another thing is TMB substrate is highly sensitive - will changing the substrate have any effect ?

In Topic: SDS-PAGE running strangely

20 August 2012 - 05:23 PM

u can confirm protein by Western blot and forget about how it migrates.

Sometimes proteins dont migrate just based on their MW - protein characteristics affect the migration

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