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Ben Lomond

Member Since 29 May 2011
Offline Last Active May 16 2013 04:55 PM
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Posts I've Made

In Topic: Re use the pre coated microtiter plate 96 wells

22 April 2013 - 05:16 PM

It certainly would not be a recommended approach to re-use an ELISA plate from a kit.  If the reagents are stripped off using acid (1 M acetic acid for example) there may be some binding activity left, but the assay performance is likely to be changed so that the dynamic range is likely to be shifted and precision is likely to be widened.   If the kit is obtainded from a reputable manufacturer the cost of the kit is a reflection of the manufacturer's research and effort put into developing the kit so that you can provide quality research data. The dollars that you might save by trying to reuse these plates will work against the quality of your research.

Good luck!

In Topic: ELISA, BDNF, Biotinylated Conjugate

29 January 2013 - 04:41 PM

So what matrix are you using to dilute your recombinant human BDNF in?

In Topic: ELISA, BDNF, Biotinylated Conjugate

23 January 2013 - 06:51 PM

Have you been working with only one serum lot?  Many humans have circulating antibodies specific for animal immunoglobulins, and can form a bridge between the capture and detection antibody especially if both capture and detection antibodies are from the same species. For such an assay format, it is standard practice to include either mouse serum in the sample diluent to saturate human anti-mouse antibodies within the sample or to use a heterophilic blocking reagent.  Scantibodies has such products and has detailed information on their use.
Do you get a similar level of background among multiple lots of human matrix?

In Topic: What is the difference between measurment range and indication range in ELISA re

10 January 2013 - 07:24 PM

I suspect that the number is linear with colour intensity over the range 0 to 3, and less accurate between 3 and 3.5.  In other words, don't go above 3 units for quantitative assays

In Topic: Biotinylated BDNF

08 January 2013 - 04:33 PM

Tell us exactly what your capture antibody is, what you biotinylated detection antibody is, the diluents that you are using, and how you are applying your streptavidin conjugate, the blockers you are using etc. Without this information, it is difficult to provide constructive advice

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